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antibody rabbit polyclonal anti mat2a novus  (Novus Biologicals)


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    Structured Review

    Novus Biologicals antibody rabbit polyclonal anti mat2a novus
    Antibody Rabbit Polyclonal Anti Mat2a Novus, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+rabbit+polyclonal+anti+mat2a+novus/MAT2A+Antibody+-+BSA+Free/10__7554_slash_elife__64930-404-59-63
    Average 93 stars, based on 13 article reviews
    antibody rabbit polyclonal anti mat2a novus - by Bioz Stars, 2026-10
    93/100 stars

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    Recombinant:

    Article Title: SAM homeostasis is regulated by CFIm-mediated splicing of MAT2A
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (E. coli) Bacterial: ElectroMAX Stbl4 Competent Cells Thermo Fisher Cat#: 11635018 Electrocompetent Cells Strain, strain background (E. coli) Bacterial: Rosetta (DE3) cells EMD Millipore Cat#: 70954 For production of SUMO-CFIm25 Cell line (H. sapiens) HEK293 Dr. Joan A. Steitz (Yale University) Conrad and Steitz, 2005 Cell line (H. sapiens) HEK293T Dr. Joshua Mendell UT Southwestern Medical Center Cell line (H. sapiens) 293A-TOA Dr. Nicholas K. Conrad UT Southwestern Medical Center (Sahin et al., 2010) Cell line (H. sapiens) HCT116 ATCC CCL-247 Cell line (H. sapiens) HCT116-GFP-b-MAT8-3 ́ This paper ‘Reporter’ Maintained by Nicholas K. Conrad lab Cell line (H. sapiens) HCT116-DDetainedIntron (116-DDI) This paper ‘116-DDI’ Maintained by Nicholas K. Conrad lab Continued on next page Scarborough et al. eLife 2021;10:e64930. .. DOI: https://doi.org/10.7554/eLife.64930 18 of 38 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (H. sapiens) HCT116-GFP-T2Ab-MAT8-3 ́-hp2-6m9#1 This paper ‘T2A, hp2-6m9#1’ Maintained by Nicholas K. Conrad lab Cell line (H. sapiens) HCT116-GFP-T2Ab-MAT8-3 ́-hp2-6m9#2 This paper ‘T2A, hp2-6m9#2’ Maintained by Nicholas K. Conrad lab Antibody Rabbit polyclonal anti-GFP Abcam Cat#: ab6556; RRID:AB_305564 (1:2000) Antibody Rabbit polyclonal anti-MAT2A Novus Cat#: NB110-94158; RRID:AB_1237164 (1:2000) Antibody Mous monoclonal anti-b-actin Abcam Cat# ab6276; RRID:AB_2223210 (1:5000) Antibody Rabbit polyclonal anti-METTL16 Bethyl Cat# A304-192A; RRID:AB_2620389 (1:5000) Antibody Rabbit monoclonal anti-CFIm25 Invitrogen Cat#: 702871; RRID:AB_2723420 (1:2000) Antibody Rabbit polyclonal anti-CFIm59 Bethyl Cat# A301-360A; RRID:AB_937864 (1:2000) Antibody Rabbit polyclonal anti-CFIm68 Bethyl Cat#: A301-358A; RRID:AB_937785 (1:2000) Antibody Rabbit polyclonal anti-flag Sigma Cat#: F7425; RRID:AB_439687 (1:5000) Antibody Mouse monoclonal anti-Bacteriophage MS2 Coat Protein Kerafast Cat#: ED0005 (1:2000) Antibody Mouse monoclonal anti-flag Sigma Cat#: F3165; RRID:AB_259529 (1:2000) Antibody Goat anti-mouse IRDye Cat#:926–68020; RRID:AB_10706161 (1:10,000) Antibody Goat anti-mouse IRDye Cat#: 926–32210; RRID:AB_621842 (1:10,000) Antibody Goat anti-rabbit IRDye Cat#: 926–32211; RRID:AB_621843 (1:10,000) Recombinant DNA reagent Plasmid: pcDNA-flag Sahin et al., 2010 N/A Recombinant DNA reagent Plasmid: pcMS2-NLS-flag Sahin et al., 2010 N/A Recombinant DNA reagent Plasmid: b-MAT-WT Pendleton et al., 2017 ‘WT’ Recombinant DNA reagent Plasmid: b-MATpreHP1-UGCU This paper ‘UGCU’ Contains UGUU upstream of hp1 to UGCU mutation in b-MAT-WT backbone. ..

    Plasmid Preparation:

    Article Title: SAM homeostasis is regulated by CFIm-mediated splicing of MAT2A
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (E. coli) Bacterial: ElectroMAX Stbl4 Competent Cells Thermo Fisher Cat#: 11635018 Electrocompetent Cells Strain, strain background (E. coli) Bacterial: Rosetta (DE3) cells EMD Millipore Cat#: 70954 For production of SUMO-CFIm25 Cell line (H. sapiens) HEK293 Dr. Joan A. Steitz (Yale University) Conrad and Steitz, 2005 Cell line (H. sapiens) HEK293T Dr. Joshua Mendell UT Southwestern Medical Center Cell line (H. sapiens) 293A-TOA Dr. Nicholas K. Conrad UT Southwestern Medical Center (Sahin et al., 2010) Cell line (H. sapiens) HCT116 ATCC CCL-247 Cell line (H. sapiens) HCT116-GFP-b-MAT8-3 ́ This paper ‘Reporter’ Maintained by Nicholas K. Conrad lab Cell line (H. sapiens) HCT116-DDetainedIntron (116-DDI) This paper ‘116-DDI’ Maintained by Nicholas K. Conrad lab Continued on next page Scarborough et al. eLife 2021;10:e64930. .. DOI: https://doi.org/10.7554/eLife.64930 18 of 38 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (H. sapiens) HCT116-GFP-T2Ab-MAT8-3 ́-hp2-6m9#1 This paper ‘T2A, hp2-6m9#1’ Maintained by Nicholas K. Conrad lab Cell line (H. sapiens) HCT116-GFP-T2Ab-MAT8-3 ́-hp2-6m9#2 This paper ‘T2A, hp2-6m9#2’ Maintained by Nicholas K. Conrad lab Antibody Rabbit polyclonal anti-GFP Abcam Cat#: ab6556; RRID:AB_305564 (1:2000) Antibody Rabbit polyclonal anti-MAT2A Novus Cat#: NB110-94158; RRID:AB_1237164 (1:2000) Antibody Mous monoclonal anti-b-actin Abcam Cat# ab6276; RRID:AB_2223210 (1:5000) Antibody Rabbit polyclonal anti-METTL16 Bethyl Cat# A304-192A; RRID:AB_2620389 (1:5000) Antibody Rabbit monoclonal anti-CFIm25 Invitrogen Cat#: 702871; RRID:AB_2723420 (1:2000) Antibody Rabbit polyclonal anti-CFIm59 Bethyl Cat# A301-360A; RRID:AB_937864 (1:2000) Antibody Rabbit polyclonal anti-CFIm68 Bethyl Cat#: A301-358A; RRID:AB_937785 (1:2000) Antibody Rabbit polyclonal anti-flag Sigma Cat#: F7425; RRID:AB_439687 (1:5000) Antibody Mouse monoclonal anti-Bacteriophage MS2 Coat Protein Kerafast Cat#: ED0005 (1:2000) Antibody Mouse monoclonal anti-flag Sigma Cat#: F3165; RRID:AB_259529 (1:2000) Antibody Goat anti-mouse IRDye Cat#:926–68020; RRID:AB_10706161 (1:10,000) Antibody Goat anti-mouse IRDye Cat#: 926–32210; RRID:AB_621842 (1:10,000) Antibody Goat anti-rabbit IRDye Cat#: 926–32211; RRID:AB_621843 (1:10,000) Recombinant DNA reagent Plasmid: pcDNA-flag Sahin et al., 2010 N/A Recombinant DNA reagent Plasmid: pcMS2-NLS-flag Sahin et al., 2010 N/A Recombinant DNA reagent Plasmid: b-MAT-WT Pendleton et al., 2017 ‘WT’ Recombinant DNA reagent Plasmid: b-MATpreHP1-UGCU This paper ‘UGCU’ Contains UGUU upstream of hp1 to UGCU mutation in b-MAT-WT backbone. ..

    Mutagenesis:

    Article Title: SAM homeostasis is regulated by CFIm-mediated splicing of MAT2A
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (E. coli) Bacterial: ElectroMAX Stbl4 Competent Cells Thermo Fisher Cat#: 11635018 Electrocompetent Cells Strain, strain background (E. coli) Bacterial: Rosetta (DE3) cells EMD Millipore Cat#: 70954 For production of SUMO-CFIm25 Cell line (H. sapiens) HEK293 Dr. Joan A. Steitz (Yale University) Conrad and Steitz, 2005 Cell line (H. sapiens) HEK293T Dr. Joshua Mendell UT Southwestern Medical Center Cell line (H. sapiens) 293A-TOA Dr. Nicholas K. Conrad UT Southwestern Medical Center (Sahin et al., 2010) Cell line (H. sapiens) HCT116 ATCC CCL-247 Cell line (H. sapiens) HCT116-GFP-b-MAT8-3 ́ This paper ‘Reporter’ Maintained by Nicholas K. Conrad lab Cell line (H. sapiens) HCT116-DDetainedIntron (116-DDI) This paper ‘116-DDI’ Maintained by Nicholas K. Conrad lab Continued on next page Scarborough et al. eLife 2021;10:e64930. .. DOI: https://doi.org/10.7554/eLife.64930 18 of 38 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (H. sapiens) HCT116-GFP-T2Ab-MAT8-3 ́-hp2-6m9#1 This paper ‘T2A, hp2-6m9#1’ Maintained by Nicholas K. Conrad lab Cell line (H. sapiens) HCT116-GFP-T2Ab-MAT8-3 ́-hp2-6m9#2 This paper ‘T2A, hp2-6m9#2’ Maintained by Nicholas K. Conrad lab Antibody Rabbit polyclonal anti-GFP Abcam Cat#: ab6556; RRID:AB_305564 (1:2000) Antibody Rabbit polyclonal anti-MAT2A Novus Cat#: NB110-94158; RRID:AB_1237164 (1:2000) Antibody Mous monoclonal anti-b-actin Abcam Cat# ab6276; RRID:AB_2223210 (1:5000) Antibody Rabbit polyclonal anti-METTL16 Bethyl Cat# A304-192A; RRID:AB_2620389 (1:5000) Antibody Rabbit monoclonal anti-CFIm25 Invitrogen Cat#: 702871; RRID:AB_2723420 (1:2000) Antibody Rabbit polyclonal anti-CFIm59 Bethyl Cat# A301-360A; RRID:AB_937864 (1:2000) Antibody Rabbit polyclonal anti-CFIm68 Bethyl Cat#: A301-358A; RRID:AB_937785 (1:2000) Antibody Rabbit polyclonal anti-flag Sigma Cat#: F7425; RRID:AB_439687 (1:5000) Antibody Mouse monoclonal anti-Bacteriophage MS2 Coat Protein Kerafast Cat#: ED0005 (1:2000) Antibody Mouse monoclonal anti-flag Sigma Cat#: F3165; RRID:AB_259529 (1:2000) Antibody Goat anti-mouse IRDye Cat#:926–68020; RRID:AB_10706161 (1:10,000) Antibody Goat anti-mouse IRDye Cat#: 926–32210; RRID:AB_621842 (1:10,000) Antibody Goat anti-rabbit IRDye Cat#: 926–32211; RRID:AB_621843 (1:10,000) Recombinant DNA reagent Plasmid: pcDNA-flag Sahin et al., 2010 N/A Recombinant DNA reagent Plasmid: pcMS2-NLS-flag Sahin et al., 2010 N/A Recombinant DNA reagent Plasmid: b-MAT-WT Pendleton et al., 2017 ‘WT’ Recombinant DNA reagent Plasmid: b-MATpreHP1-UGCU This paper ‘UGCU’ Contains UGUU upstream of hp1 to UGCU mutation in b-MAT-WT backbone. ..



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    93
    Novus Biologicals antibody rabbit polyclonal anti mat2a novus
    Antibody Rabbit Polyclonal Anti Mat2a Novus, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+rabbit+polyclonal+anti+mat2a+novus/MAT2A+Antibody+-+BSA+Free/10__7554_slash_elife__64930-404-59-63
    Average 93 stars, based on 1 article reviews
    antibody rabbit polyclonal anti mat2a novus - by Bioz Stars, 2026-10
    93/100 stars
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